1. ZHAW digitalcollection
  2. School of Engineering
  3. Publikationen
  4. Publikationen School of Engineering
Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-25992
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNytko, Katarzyna J.-
dc.contributor.authorThumser-Henner, Pauline-
dc.contributor.authorRusso, Giancarlo-
dc.contributor.authorWeyland, Mathias S.-
dc.contributor.authorRohrer Bley, Carla-
dc.date.accessioned2022-11-11T09:45:32Z-
dc.date.available2022-11-11T09:45:32Z-
dc.date.issued2020-
dc.identifier.issn2045-2322de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/25992-
dc.description.abstractPre-treatment of tumors with hyperthermia is often used to increase the efficacy of radiotherapy. One of the main proteins induced in response to hyperthermia is heat shock protein 70 (HSP70). The aim of our study was to investigate up- and down-regulated genes in response to (thermo)radiotherapy in HSP70 proficient and deficient canine osteosarcoma cell line (Abrams), and functional role of HSP70 in the mechanism of thermoradiosensitization. Cells were transfected with negative control siRNA or siRNA targeting HSP70 and treated with hyperthermia (HT), radiotherapy (RT), and thermoradiotherapy (HTRT). RNA sequencing was used to analyze gene expression. Hyperthermia and thermoradiotherapy, but not radiotherapy alone, induced differential gene expression. We identified genes differentially expressed only in HSP70 knockdown (thus HSP70-dependent) cells in response to hyperthermia and thermoradiotherapy. Interestingly, cell proliferation but not clonogenicity and apoptosis/necrosis was affected by the HSP70 knockdown in response to thermoradiotherapy. The results suggest that HSP70 regulates expression of specific genes in response to hyperthermia and thermoradiotherapy. Further investigations into the role of specific genes regulated in a HSP70-dependent manner in response to thermoradiotherapy could pave a way into new, combinatorial treatment options for (canine) osteosarcoma and other cancer types.de_CH
dc.language.isoende_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofScientific Reportsde_CH
dc.rightshttps://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectAnimalde_CH
dc.subjectBone neoplasmde_CH
dc.subjectCell proliferationde_CH
dc.subjectCluster analysisde_CH
dc.subjectDisease model, animalde_CH
dc.subjectDogde_CH
dc.subjectGene expression regulation, neoplasticde_CH
dc.subjectHSP70 heat-shock proteinde_CH
dc.subjectHyperthermia, inducedde_CH
dc.subjectMatrix metalloproteinase 1de_CH
dc.subjectMembrane glycoproteinde_CH
dc.subjectOsteosarcomade_CH
dc.subjectPhotonde_CH
dc.subjectPrincipal component analysisde_CH
dc.subjectRNA, messengerde_CH
dc.subjectRNA, small interferingde_CH
dc.subjectRNA-seqde_CH
dc.subjectRadiotherapyde_CH
dc.subjectVesicular transport proteinde_CH
dc.subject.ddc572: Biochemiede_CH
dc.subject.ddc615: Pharmakologie und Therapeutikde_CH
dc.titleRole of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seqde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.organisationalunitInstitut für Angewandte Mathematik und Physik (IAMP)de_CH
dc.identifier.doi10.1038/s41598-020-69619-2de_CH
dc.identifier.doi10.21256/zhaw-25992-
dc.identifier.pmid32728031de_CH
zhaw.funding.euNode_CH
zhaw.issue1de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.start12779de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume10de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen School of Engineering

Files in This Item:
File Description SizeFormat 
2020_Nytko-etal_Role-of-HSP70-inresponse-to-thermoradiotherapie_Sci-Rep.pdf1.59 MBAdobe PDFThumbnail
View/Open
Show simple item record
Nytko, K. J., Thumser-Henner, P., Russo, G., Weyland, M. S., & Rohrer Bley, C. (2020). Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq. Scientific Reports, 10(1), 12779. https://doi.org/10.1038/s41598-020-69619-2
Nytko, K.J. et al. (2020) ‘Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq’, Scientific Reports, 10(1), p. 12779. Available at: https://doi.org/10.1038/s41598-020-69619-2.
K. J. Nytko, P. Thumser-Henner, G. Russo, M. S. Weyland, and C. Rohrer Bley, “Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq,” Scientific Reports, vol. 10, no. 1, p. 12779, 2020, doi: 10.1038/s41598-020-69619-2.
NYTKO, Katarzyna J., Pauline THUMSER-HENNER, Giancarlo RUSSO, Mathias S. WEYLAND und Carla ROHRER BLEY, 2020. Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq. Scientific Reports. 2020. Bd. 10, Nr. 1, S. 12779. DOI 10.1038/s41598-020-69619-2
Nytko, Katarzyna J., Pauline Thumser-Henner, Giancarlo Russo, Mathias S. Weyland, and Carla Rohrer Bley. 2020. “Role of HSP70 in Response to (Thermo)radiotherapy : Analysis of Gene Expression in Canine Osteosarcoma Cells by RNA-Seq.” Scientific Reports 10 (1): 12779. https://doi.org/10.1038/s41598-020-69619-2.
Nytko, Katarzyna J., et al. “Role of HSP70 in Response to (Thermo)radiotherapy : Analysis of Gene Expression in Canine Osteosarcoma Cells by RNA-Seq.” Scientific Reports, vol. 10, no. 1, 2020, p. 12779, https://doi.org/10.1038/s41598-020-69619-2.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.