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https://doi.org/10.21256/zhaw-26862| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes |
| Authors: | Müller, Jan Ott, Vivian Weiss, Noémi Neubauer, Peter Eibl, Dieter Eibl, Regine |
| et. al: | No |
| DOI: | 10.1002/cite.202200097 10.21256/zhaw-26862 |
| Published in: | Chemie Ingenieur Technik |
| Volume(Issue): | 94 |
| Issue: | 12 |
| Page(s): | 1977 |
| Pages to: | 1984 |
| Issue Date: | 17-Oct-2022 |
| Publisher / Ed. Institution: | Wiley |
| ISSN: | 0009-286X 1522-2640 |
| Language: | English |
| Subjects: | Chinese hamster ovary cell; Monoclonal antibody; N-1 perfusion; Ultra-high cell density |
| Subject (DDC): | 660.6: Biotechnology |
| Abstract: | Various approaches to process intensification are currently being investigated to ensure time and cost savings when producing biopharmaceuticals. In the present study, a one-step inoculum production was established based on an ultra-high cell density working cell bank with immunoglobulin G producing Chinese hamster ovary cells. Cryovials were used for direct inoculation of 1-L wave-mixed perfusion bioreactors. When reaching around 180 · 106 cells mL−1 in N−1 perfusion mode, low-seed and high-seed fed-batch experiments in shake flasks were inoculated, as was the case for 250-mL stirred single-use bioreactors. Additionally, proof-of-concept runs at 50-L and 200-L scale were successfully performed. The intensification approach presented allows manufacturing capacity to be increased by up to 50 %. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/26862 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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| 2022_Mueller-etal_Process-intensification-one‐step-inoculum-production.pdf | 461.14 kB | Adobe PDF |  View/Open |
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Müller, J., Ott, V., Weiss, N., Neubauer, P., Eibl, D., & Eibl, R. (2022). Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes. Chemie Ingenieur Technik, 94(12), 1977–1984. https://doi.org/10.1002/cite.202200097
Müller, J. et al. (2022) ‘Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes’, Chemie Ingenieur Technik, 94(12), pp. 1977–1984. Available at: https://doi.org/10.1002/cite.202200097.
J. Müller, V. Ott, N. Weiss, P. Neubauer, D. Eibl, and R. Eibl, “Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes,” Chemie Ingenieur Technik, vol. 94, no. 12, pp. 1977–1984, Oct. 2022, doi: 10.1002/cite.202200097.
MÜLLER, Jan, Vivian OTT, Noémi WEISS, Peter NEUBAUER, Dieter EIBL und Regine EIBL, 2022. Process intensification using a one‐step inoculum production and high‐seeded fed‐batch processes. Chemie Ingenieur Technik. 17 Oktober 2022. Bd. 94, Nr. 12, S. 1977–1984. DOI 10.1002/cite.202200097
Müller, Jan, Vivian Ott, Noémi Weiss, Peter Neubauer, Dieter Eibl, and Regine Eibl. 2022. “Process Intensification Using a One‐Step Inoculum Production and High‐Seeded Fed‐Batch Processes.” Chemie Ingenieur Technik 94 (12): 1977–84. https://doi.org/10.1002/cite.202200097.
Müller, Jan, et al. “Process Intensification Using a One‐Step Inoculum Production and High‐Seeded Fed‐Batch Processes.” Chemie Ingenieur Technik, vol. 94, no. 12, Oct. 2022, pp. 1977–84, https://doi.org/10.1002/cite.202200097.
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