Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture

Rampin, Andrea; Skoufos, Ioannis; Raghunath, Michael; Tzora, Athina; Diakakis, Nikolaos; Prassinos, Nikitas; Zeugolis, Dimitrios I.

doi:10.3390/cells11091562

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26891

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture
Authors:	Rampin, Andrea Skoufos, Ioannis Raghunath, Michael Tzora, Athina Diakakis, Nikolaos Prassinos, Nikitas Zeugolis, Dimitrios I.
et. al:	No
DOI:	10.3390/cells11091562 10.21256/zhaw-26891
Published in:	Cells
Volume(Issue):	11
Issue:	9
Page(s):	1562
Issue Date:	5-May-2022
Publisher / Ed. Institution:	MDPI
ISSN:	2073-4409
Language:	English
Subjects:	Allogeneic serum; Excluded volume effect; Foetal bovine serum; Serial passaging; Tenocyte aging; Tenocyte function; Animal; Extracellular matrix; Horse; Macromolecular substance; Serum albumin, bovine; Hematopoietic stem cell transplantation; Tenocyte
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	The absence of a native extracellular matrix and the use of xenogeneic sera are often associated with rapid tenocyte function losses during in vitro culture. Herein, we assessed the influence of different sera (equine serum and foetal bovine serum) on equine tenocyte morphology, viability, metabolic activity, proliferation and protein synthesis as a function of tissue-specific extracellular matrix deposition (induced via macromolecular crowding), aging (passages 3, 6, 9) and time in culture (days 3, 5, 7). In comparison to cells at passage 3, at day 3, in foetal bovine serum and without macromolecular crowding (traditional equine tenocyte culture), the highest number of significantly decreased readouts were observed for cells in foetal bovine serum, at passage 3, at day 5 and day 7 and without macromolecular crowding. Again, in comparison to traditional equine tenocyte culture, the highest number of significantly increased readouts were observed for cells in equine serum, at passage 3 and passage 6, at day 7 and with macromolecular crowding. Our data advocate the use of an allogeneic serum and tissue-specific extracellular matrix for effective expansion of equine tenocytes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26891
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Rampin, A., Skoufos, I., Raghunath, M., Tzora, A., Diakakis, N., Prassinos, N., & Zeugolis, D. I. (2022). Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture. Cells, 11(9), 1562. https://doi.org/10.3390/cells11091562

Rampin, A. et al. (2022) ‘Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture’, Cells, 11(9), p. 1562. Available at: https://doi.org/10.3390/cells11091562.

A. Rampin et al., “Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture,” Cells, vol. 11, no. 9, p. 1562, May 2022, doi: 10.3390/cells11091562.

RAMPIN, Andrea, Ioannis SKOUFOS, Michael RAGHUNATH, Athina TZORA, Nikolaos DIAKAKIS, Nikitas PRASSINOS und Dimitrios I. ZEUGOLIS, 2022. Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture. Cells. 5 Mai 2022. Bd. 11, Nr. 9, S. 1562. DOI 10.3390/cells11091562

Rampin, Andrea, Ioannis Skoufos, Michael Raghunath, Athina Tzora, Nikolaos Diakakis, Nikitas Prassinos, and Dimitrios I. Zeugolis. 2022. “Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.” Cells 11 (9): 1562. https://doi.org/10.3390/cells11091562.

Rampin, Andrea, et al. “Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.” Cells, vol. 11, no. 9, May 2022, p. 1562, https://doi.org/10.3390/cells11091562.