Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect

Assunção, Marisa; Wong, Christy Wingtung; Richardson, Joseph J.; Tsang, Rachel; Beyer, Sebastian; Raghunath, Michael; Blocki, Anna

doi:10.1016/j.msec.2019.110280

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect
Authors:	Assunção, Marisa Wong, Christy Wingtung Richardson, Joseph J. Tsang, Rachel Beyer, Sebastian Raghunath, Michael Blocki, Anna
et. al:	No
DOI:	10.1016/j.msec.2019.110280
Published in:	Materials Science and Engineering C: Materials for Biological Applications
Volume(Issue):	106
Issue:	110280
Issue Date:	2020
Publisher / Ed. Institution:	Elsevier
ISSN:	0928-4931
Language:	English
Subjects:	Dextran sulfate; Extracellular matrix deposition; Macromolecular crowding; Mesenchymal stromal cell; Microenvironment; Tissue engineering; Adipogenesis; Bone marrow cell; Cell differentiation; Cells, cultured; Collagen type I; Culture media; Dextran sulfate; Extracellular matrix; Fibronectins; Humans; Mesenchymal stem Cell; Osteogenesis; Static clectricity
Subject (DDC):	610.28: Biomedicine, biomedical engineering
Abstract:	A faithful reconstruction of the native cellular microenvironment is instrumental for tissue engineering. Macromolecular crowding (MMC) empowers cells to deposit their own extracellular matrix (ECM) in greater amounts, and thus contributes to building tissue-specific complex microenvironments in vitro. Dextran sulfate (DxS, 500 kDa), a semi-synthetic sulfated polyglucose, was shown previously at a fractional volume occupancy (FVO) of 5.2% (v/v; 100 μg/ml) to act as a potent molecular crowding agent in vitro. When added to human mesenchymal stromal cell (MSC) cultures, DxS enhanced fibronectin and collagen I deposition several-fold also at concentrations with negligible FVO (<1% v/v). In a cell-free system, incubation of culture media supplemented with fetal bovine serum (FBS), purified fibronectin or collagen I with DxS led to a co-deposition of respective components, exhibiting a similar granular pattern as observed in cell culture. Aggregation of FBS components, fibronectin or collagen I with DxS was confirmed by dynamic light scattering, where an increase in hydrodynamic radius in the respective mixtures was observed. FBS- and fibronectin aggregates could be dissociated with increasing salt concentrations, indicating electrostatic forces to be responsible for the aggregation. Conversely, collagen I-DxS aggregates increased in size with increasing ion concentration, likely caused by charge screening of collagen I, which is net negatively charged at neutral pH, thus permitting weaker intermolecular interactions to occur. The incorporation of DxS into the ECM resulted in altered ECM topography and stiffness. DxS-supplemented cultures exhibited potentiated bioactivity, such as enhanced adipogenic and especially osteogenic differentiation under inductive conditions. We propose an alternative mechanism by which DxS drives ECM deposition via aggregation, and in an independent manner from MMC. A deeper understanding of the underlying mechanism will enable optimized engineering approaches for ECM-rich tissue constructs.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26922
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Assunção, M., Wong, C. W., Richardson, J. J., Tsang, R., Beyer, S., Raghunath, M., & Blocki, A. (2020). Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect. Materials Science and Engineering C: Materials for Biological Applications, 106(110280). https://doi.org/10.1016/j.msec.2019.110280

Assunção, M. et al. (2020) ‘Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect’, Materials Science and Engineering C: Materials for Biological Applications, 106(110280). Available at: https://doi.org/10.1016/j.msec.2019.110280.

M. Assunção et al., “Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect,” Materials Science and Engineering C: Materials for Biological Applications, vol. 106, no. 110280, 2020, doi: 10.1016/j.msec.2019.110280.

ASSUNÇÃO, Marisa, Christy Wingtung WONG, Joseph J. RICHARDSON, Rachel TSANG, Sebastian BEYER, Michael RAGHUNATH und Anna BLOCKI, 2020. Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect. Materials Science and Engineering C: Materials for Biological Applications. 2020. Bd. 106, Nr. 110280. DOI 10.1016/j.msec.2019.110280

Assunção, Marisa, Christy Wingtung Wong, Joseph J. Richardson, Rachel Tsang, Sebastian Beyer, Michael Raghunath, and Anna Blocki. 2020. “Macromolecular Dextran Sulfate Facilitates Extracellular Matrix Deposition by Electrostatic Interaction Independent from a Macromolecular Crowding Effect.” Materials Science and Engineering C: Materials for Biological Applications 106 (110280). https://doi.org/10.1016/j.msec.2019.110280.

Assunção, Marisa, et al. “Macromolecular Dextran Sulfate Facilitates Extracellular Matrix Deposition by Electrostatic Interaction Independent from a Macromolecular Crowding Effect.” Materials Science and Engineering C: Materials for Biological Applications, vol. 106, no. 110280, 2020, https://doi.org/10.1016/j.msec.2019.110280.