The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis

Eichenberger, Michael; Schwander, Thomas; Hüppi, Sean; Kreuzer, Jan; Mittl, Peer R. E.; Peccati, Francesca; Jiménez-Osés, Gonzalo; Naesby, Michael; Buller, Rebecca M.

doi:10.1038/s41929-023-01018-y

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-29458

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis
Authors:	Eichenberger, Michael Schwander, Thomas Hüppi, Sean Kreuzer, Jan Mittl, Peer R. E. Peccati, Francesca Jiménez-Osés, Gonzalo Naesby, Michael Buller, Rebecca M.
et. al:	No
DOI:	10.1038/s41929-023-01018-y 10.21256/zhaw-29458
Published in:	Nature Catalysis
Volume(Issue):	6
Issue:	10
Page(s):	927
Pages to:	938
Issue Date:	2023
Publisher / Ed. Institution:	Nature Publishing Group
ISSN:	2520-1158
Language:	English
Subjects:	Biocatalysis; Mechanism of action; Synthetic biology; X-ray crystallography
Subject (DDC):	660.6: Biotechnology
Abstract:	Anthocyanins are ubiquitous plant pigments used in a variety of technological applications. Yet, after over a century of research, the penultimate biosynthetic step to anthocyanidins attributed to the action of leucoanthocyanidin dioxygenase has never been efficiently reconstituted outside plants, preventing the construction of heterologous cell factories. Through biochemical and structural analysis, here we show that anthocyanin-related glutathione transferases, currently implicated only in anthocyanin transport, catalyse an essential dehydration of the leucoanthocyanidin dioxygenase product, flavan-3,3,4-triol, to generate cyanidin. Building on this knowledge, introduction of anthocyanin-related glutathione transferases into a heterologous biosynthetic pathway in baker's yeast results in >35-fold increased anthocyanin production. In addition to unravelling the long-elusive anthocyanin biosynthesis, our findings pave the way for the colourants' heterologous microbial production and could impact the breeding of industrial and ornamental plants.
URI:	https://digitalcollection.zhaw.ch/handle/11475/29458
Related research data:	https://doi.org/10.2210/pdb8agq/pdb https://doi.org/10.2210/pdb5f07/pdb https://doi.org/10.5281/zenodo.8069429
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Eichenberger, M., Schwander, T., Hüppi, S., Kreuzer, J., Mittl, P. R. E., Peccati, F., Jiménez-Osés, G., Naesby, M., & Buller, R. M. (2023). The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis. Nature Catalysis, 6(10), 927–938. https://doi.org/10.1038/s41929-023-01018-y

Eichenberger, M. et al. (2023) ‘The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis’, Nature Catalysis, 6(10), pp. 927–938. Available at: https://doi.org/10.1038/s41929-023-01018-y.

M. Eichenberger et al., “The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis,” Nature Catalysis, vol. 6, no. 10, pp. 927–938, 2023, doi: 10.1038/s41929-023-01018-y.

EICHENBERGER, Michael, Thomas SCHWANDER, Sean HÜPPI, Jan KREUZER, Peer R. E. MITTL, Francesca PECCATI, Gonzalo JIMÉNEZ-OSÉS, Michael NAESBY und Rebecca M. BULLER, 2023. The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis. Nature Catalysis. 2023. Bd. 6, Nr. 10, S. 927–938. DOI 10.1038/s41929-023-01018-y

Eichenberger, Michael, Thomas Schwander, Sean Hüppi, Jan Kreuzer, Peer R. E. Mittl, Francesca Peccati, Gonzalo Jiménez-Osés, Michael Naesby, and Rebecca M. Buller. 2023. “The Catalytic Role of Glutathione Transferases in Heterologous Anthocyanin Biosynthesis.” Nature Catalysis 6 (10): 927–38. https://doi.org/10.1038/s41929-023-01018-y.

Eichenberger, Michael, et al. “The Catalytic Role of Glutathione Transferases in Heterologous Anthocyanin Biosynthesis.” Nature Catalysis, vol. 6, no. 10, 2023, pp. 927–38, https://doi.org/10.1038/s41929-023-01018-y.