Publication type: Article in scientific journal
Type of review: Not specified
Title: Engineering fire blight resistance into the apple cultivar 'Gala' using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni
Authors: Broggini, Giovanni A. L.
Wöhner, Thomas
Fahrentrapp, Johannes
Kost, Thomas D.
Flachowsky, Henryk
Peil, Andreas
Hanke, Maria-Viola
Richter, Klaus
Patocchi, Andrea
Gessler, Cesare
DOI: 10.1111/pbi.12177
Published in: Plant Biotechnology Journal
Volume(Issue): 12
Issue: 6
Page(s): 728
Pages to: 733
Issue Date: Mar-2014
Publisher / Ed. Institution: Wiley
ISSN: 1467-7644
Language: English
Subjects: Genetic engineering; GMO; Resistance; Weinbau
Subject (DDC): 634: Orchards, fruits and forestry
660.6: Biotechnology
Abstract: The fire blight susceptible apple cultivar Malus × domestica Borkh. cv. ‘Gala’ was transformed with the candidate fire blight resistance gene FB_MR5 originating from the crab apple accession Malus × robusta 5 (Mr5). A total of five different transgenic lines were obtained. All transgenic lines were shown to be stably transformed and originate from different transgenic events. The transgenic lines express the FB_MR5 either driven by the constitutive CaMV 35S promoter and the ocs terminator or by its native promoter and terminator sequences. Phenotyping experiments were performed with Mr5-virulent and Mr5-avirulent strains of Erwinia amylovora, the causal agent of fire blight. Significantly less disease symptoms were detected on transgenic lines after inoculation with two different Mr5-avirulent E. amylovora strains, while significantly more shoot necrosis was observed after inoculation with the Mr5-virulent mutant strain ZYRKD3_1. The results of these experiments demonstrated the ability of a single gene isolated from the native gene pool of apple to protect a susceptible cultivar from fire blight. Furthermore, this gene is confirmed to be the resistance determinant of Mr5 as the transformed lines undergo the same gene-for-gene interaction in the host–pathogen relationship Mr5–E. amylovora.
Fulltext version: Published version
License (according to publishing contract): Licence according to publishing contract
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project: Occurence of grape phylloxera (Dactulosphaira vitifoliae) in Switzerland
Appears in collections:Publikationen Life Sciences und Facility Management

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