A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts

Mathes, Stephanie H.; Wohlwend, Lorenz; Uebersax, Lorenz; von Mentlen, Roger; Thoma, Daniel S.; Jung, Ronald E.; Görlach, Christoph; Graf-Hausner, Ursula

doi:10.1002/bit.22893

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts
Authors:	Mathes, Stephanie H. Wohlwend, Lorenz Uebersax, Lorenz von Mentlen, Roger Thoma, Daniel S. Jung, Ronald E. Görlach, Christoph Graf-Hausner, Ursula
DOI:	10.1002/bit.22893
Published in:	Biotechnology & Bioengineering
Volume(Issue):	107
Issue:	6
Page(s):	1029
Pages to:	1039
Issue Date:	2010
Publisher / Ed. Institution:	Wiley
ISSN:	0006-3592 1097-0290
Language:	English
Subjects:	Connective tissue; Human; Mouth mucosa; Organ culture technique; Mechanical stress; Physiological stress; Transplant; Bioreactor
Subject (DDC):	571: Physiology and related subjects 660.6: Biotechnology
Abstract:	Gingival cells of the oral connective tissue are exposed to complex mechanical forces during mastication, speech, tooth movement and orthodontic treatments. Especially during wound healing following surgical procedures, internal and external forces may occur, creating pressure upon the newly formed tissue. This clinical situation has to be considered when developing biomaterials to augment soft tissue in the oral cavity. In order to pre-evaluate a collagen sponge intended to serve as a substitute for autogenous connective tissue grafts (CTGs), a dynamic bioreactor system was developed. Pressure and shear forces can be applied in this bioreactor in addition to a constant medium perfusion to cell-material constructs. Three-dimensional volume changes and stiffness of the matrices were analyzed. In addition, cell responses such as cell vitality and extracellular matrix (ECM) production were investigated. The number of metabolic active cells constantly increased under fully dynamic culture conditions. The sponges remained elastic even after mechanical forces were applied for 14 days. Analysis of collagen type I and fibronectin revealed a statistically significant accumulation of these ECM molecules (P < 0.05-0.001) when compared to static cultures. An increased expression of tenascin-c, indicating tissue remodeling processes, was observed under dynamic conditions only. The results indicate that the tested in vitro cell culture system was able to mimic both the biological and mechanical environments of the clinical situation in a healing wound.
URI:	https://digitalcollection.zhaw.ch/handle/11475/16378
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management School of Engineering
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT) Institute of Mechanical Systems (IMES)
Appears in collections:	Publikationen School of Engineering

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Mathes, S. H., Wohlwend, L., Uebersax, L., von Mentlen, R., Thoma, D. S., Jung, R. E., Görlach, C., & Graf-Hausner, U. (2010). A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnology & Bioengineering, 107(6), 1029–1039. https://doi.org/10.1002/bit.22893

Mathes, S.H. et al. (2010) ‘A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts’, Biotechnology & Bioengineering, 107(6), pp. 1029–1039. Available at: https://doi.org/10.1002/bit.22893.

S. H. Mathes et al., “A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts,” Biotechnology & Bioengineering, vol. 107, no. 6, pp. 1029–1039, 2010, doi: 10.1002/bit.22893.

MATHES, Stephanie H., Lorenz WOHLWEND, Lorenz UEBERSAX, Roger VON MENTLEN, Daniel S. THOMA, Ronald E. JUNG, Christoph GÖRLACH und Ursula GRAF-HAUSNER, 2010. A bioreactor test system to mimic the biological and mechanical environment of oral soft tissues and to evaluate substitutes for connective tissue grafts. Biotechnology & Bioengineering. 2010. Bd. 107, Nr. 6, S. 1029–1039. DOI 10.1002/bit.22893

Mathes, Stephanie H., Lorenz Wohlwend, Lorenz Uebersax, Roger von Mentlen, Daniel S. Thoma, Ronald E. Jung, Christoph Görlach, and Ursula Graf-Hausner. 2010. “A Bioreactor Test System to Mimic the Biological and Mechanical Environment of Oral Soft Tissues and to Evaluate Substitutes for Connective Tissue Grafts.” Biotechnology & Bioengineering 107 (6): 1029–39. https://doi.org/10.1002/bit.22893.

Mathes, Stephanie H., et al. “A Bioreactor Test System to Mimic the Biological and Mechanical Environment of Oral Soft Tissues and to Evaluate Substitutes for Connective Tissue Grafts.” Biotechnology & Bioengineering, vol. 107, no. 6, 2010, pp. 1029–39, https://doi.org/10.1002/bit.22893.